Skip to main content
Powered by Drupal

Publications

World health Organization's guidance for tracking non-communicable diseases towards sustainable development goals 3.4: an initiative for facility-based monitoring

1 day 7 hours ago
BACKGROUND: Non-communicable diseases (NCDs) account for over 60% of annual global deaths, disproportionately affecting low- and middle-income countries. This trend undermines progress toward Sustainable Development Goal (SDG) 3.4, which seeks to reduce premature mortality from NCDs by one-third by 2030. Despite the availability of effective and relatively affordable interventions, addressing NCDs requires sustained, coordinated efforts and robust monitoring systems. Facility-based monitoring...
Arlene Quiambao

Prophages block cell surface receptors to preserve their viral progeny

3 days 7 hours ago
In microbial communities, viruses compete for host cells and have evolved diverse mechanisms to inhibit competitors. One strategy is superinfection exclusion, whereby an established viral infection prevents a secondary infection of the same cell¹. This phenomenon has been shown to have an important role in the spread of eukaryotic viruses. Here we determine that superinfection exclusion proteins in bacterial viruses (bacteriophages, hereafter phages) perform a similar role, promoting viral...
Véronique L Taylor

The use of machine learning models to predict progression-free survival and overall survival outcomes from waterfall plots in randomized clinical trials (MAP-OUTCOMES)

4 days 7 hours ago
CONCLUSIONS: The MAP-OUTCOMES model demonstrated the feasibility of using ML to predict survival-based outcomes from waterfall plots, thus providing a potential tool for early trial evaluation. Improving the model's performance with more training data and creating independent datasets are necessary steps to assess its generalizability for prospective clinical applications.
K Alshankati

The influence of biological sex on skeletal muscle phospholipid membrane composition in response to omega n-3 polyunsaturated fatty acid supplementation and washout in humans

4 days 7 hours ago
Omega n-3 polyunsaturated fatty acid (n-3 PUFA) intake is associated with reduced inflammation, protection against cardiovascular disease, and improved cognitive function. There is also evidence that n-3 PUFA intake impacts skeletal muscle, potentially with greater effects in females than males. Sex-specific responses to n-3 PUFA intake may be linked to the incorporation of eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) into skeletal muscle phospholipid membranes....
Callum J Pufahl

Modeling and optimization of bioproduct formation with purple phototrophic bacteria using machine learning

5 days 7 hours ago
Municipal and industrial wastewater, along with organic waste, can be transformed into valuable bioproducts using purple phototrophic bacteria. This study compares the performance of three machine learning models (Random Forest, XGBoost, and CatBoost) in predicting and optimizing the formation of key bioproducts: polyhydroxybutyrate, polyhydroxyvalerate, 5-aminolevulinic acid, coenzyme Q10, carotenoids, bacteriochlorophylls, and biomass. The models were trained on a dataset compiled from...
Germán Buitrón

<em>Dehalobacter</em> Dechlorinates Dichloroanilines and Contributes to the Natural Attenuation of Dichloronitrobenzenes at a Complex Industrial Site

5 days 7 hours ago
The potential for bioremediation of dichloroanilines (2,3- and 3,4-DCA) and dichloronitrobenzenes (3,4-, 2,5-, and 2,3-DCNB) was investigated using inocula from an industrial site in the northeast of Brazil. Anaerobic biotransformation of these chlorinated compounds was observed in microcosms simulating site conditions, particularly when an electron donor was added. To disentangle specific transformation reactions, subcultures from active anaerobic microcosms were enriched with individual DCA or...
Sofia P Araújo

Reductive Dechlorination of 2,3-Dichloroaniline by <em>Dehalobacter</em> in an Anaerobic Enrichment Culture

5 days 7 hours ago
2,3-dichloroaniline (2,3-DCA) has widespread use in chemical manufacturing and remains a persistent groundwater contaminant. To better understand the pathway and kinetics of its reductive dechlorination, we conducted a laboratory kinetic experiment using an anaerobic enrichment culture dominated by Dehalobacter. At an initial field-relevant concentration of 40 mg/L, complete and stoichiometric dechlorination of 2,3-DCA to aniline via 2-chloroaniline (2-CA) was achieved. The intermediates, 2-CA...
Shuping Wang

A role for early surgical intervention in childhood low-grade glioma? Illustrative case

5 days 7 hours ago
BACKGROUND: IDH-mutant diffuse gliomas are considered low grade, albeit with a propensity for malignant behavior over time, distinguishing them from pediatric-type low-grade gliomas. IDH-mutant tumors have astrocytic or oligodendrocytic phenotypes associated with TP53 and ATRX mutations or 1p/19q deletions, respectively. TP53 mutations are associated with a tumor that acquires malignant properties characteristic of glioblastoma with subsequent very poor survival.
Youngkyung Jung

Bacterial ADP-heptose triggers stem cell regeneration in the intestinal epithelium following injury

1 week ago
ADP-heptose (ADP-Hep), a metabolite produced by gram-negative bacteria, is detected in the host cytosol by the kinase ALPK1, which engages TIFA-dependent innate immune responses. However, the function of ALPK1-TIFA signaling in primary cells and in physiological settings remains poorly understood. Here, we showed that, in the intestinal epithelium, ALPK1 and TIFA were mainly expressed by the intestinal stem cell (ISC) pool, where they controlled the replacement of homeostatic ISCs by new revival...
Shawn Goyal

Advancing the Landscape of Clinical Actionability in Von Hippel-Lindau Syndrome: An Evidence-Based Framework from the INT<sup>2</sup>GRATE Oncology Consortium

1 week ago
Background/Objectives: An accurate evaluation of variant actionability is essential in cancer management. In Von Hippel-Lindau Syndrome (VHL), the interpretation of the germline variants is confounded by the presence of non-syndromic component tumors, such as clear cell renal cell carcinoma (ccRCC), hemangioblastoma, pheochromocytoma, and neuroendocrine tumors. These tumors frequently occur sporadically, without any association with VHL syndrome. The presence of these tumors in a patient with a...
Diane R Koeller

Enhancing the rectification effect of hydrogel-based stretchable ionic diodes through incorporating cations with high valence

1 week 1 day ago
The controlled migration of ions in biological systems has inspired the development of ion-based electronics. Ionic diodes, leveraging ions as charge carriers, offer selective control over ion flux, mimicking ion-selective behavior observed in biological systems. Conventional ionic diodes containing fluids encounter challenges in adapting to biological systems due to their limited stretchability and stability. Recent advancements in solid-state ionic diodes based on stretchable gels enable...
Pengfei Xu

2025 European LeukemiaNet recommendations for the management of chronic myeloid leukemia

1 week 1 day ago
In this 5th version of the European LeukemiaNet guidance for adult patients, there are important changes in several areas of management based on evidence available since 2020, including the World Health Organisation's reclassification of CML as a biphasic disease. Previous advice to switch the tyrosine kinase inhibitor (TKI) on failure of molecular milestones, is modified to better account for individual patient circumstances. Our recommendations are summarized in tables designed to be read in...
Jane F Apperley

Optimizing Proximity Proteomics on the EvoSep-timsTOF LC-MS System

1 week 1 day ago
Proximity-dependent biotinylation (BioID) is a powerful means of exploring the cellular environments in which proteins reside. Expressing a protein of interest (bait) fused to a biotin ligase and adding biotin induces the covalent biotinylation of proximal partners (preys), which are recovered on streptavidin beads and identified by MS. However, a major technical limitation of BioID is peptide carryover into subsequent MS runs. This is typically mitigated via lengthy intersample wash cycles,...
Julia Kitaygorodsky

Lambdoid phages with abundant Chi recombination hotspots reflect diverse viral strategies for recombination-dependent growth

1 week 2 days ago
Many phages encode recombination-mediating enzymes, but characterization of their roles in phage lifecycles is limited, and their impact on phage replication is controversial. To address these issues, we have searched for phages whose growth is impacted by the major recombination-promoting helicase-nuclease of Escherichia coli, the RecBCD enzyme. Although no phages inhibited by RecBCD are identified, growth of a newly isolated phage, named LLS, is enhanced by RecBCD. LLS's genome sequence...
Clarence Zheng

Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effects

1 week 3 days ago
Genetic variants often produce complex phenotypic effects that confound current assays and predictive models. We developed Variant in situ sequencing (VIS-seq), a pooled, image-based method that measures variant effects on molecular and cellular phenotypes in diverse cell types. Applying VIS-seq to ~3,000 LMNA and PTEN variants yielded high-dimensional morphological profiles that captured variant-driven changes in protein abundance, localization, activity and cell architecture. We identified...
Sriram Pendyala

Subgenual anterior cingulate cortex functional connectivity abnormalities in depression: insights from brain imaging big data and precision-guided personalized intervention via transcranial magnetic stimulation

1 week 4 days ago
The subgenual anterior cingulate cortex (sgACC) plays a central role in the pathophysiology of major depressive disorder (MDD). Its functional interactive profile with the left dorsal lateral prefrontal cortex (DLPFC) is associated with transcranial magnetic stimulation (TMS) treatment outcomes. Previous research on sgACC functional connectivity (FC) in MDD has yielded inconsistent results, partly due to small sample sizes and limited statistical power. Furthermore, calculating sgACC-FC to...
Xiao Chen
Checked
1 minute 13 seconds ago
Subscribe to Publications feed