Skip to main content
Powered by Drupal

Publications

Disease-specific U1 spliceosomal RNA mutations in mature B-cell neoplasms

1 day 13 hours ago
Recurrent mutations in the third base of U1 spliceosomal RNA responsible for marked splicing and expression abnormalities have been described in chronic lymphocytic leukemia (CLL) and some solid tumors. However, the clinical significance of these mutations in large and independent CLL cohorts as well as their presence in other B-cell neoplasms is unknown. Here we characterized U1 mutations in 1670 CLL and 363 mature B-cell lymphomas. We confirmed that the g.3A>C U1 mutation is found in 3.5% of...
Ferran Nadeu

Sensitive multichannel zero-to ultralow-field NMR with atomic magnetometer arrays

1 day 13 hours ago
Despite its versatility and high chemical specificity, conventional nuclear magnetic resonance (NMR) spectroscopy is limited in measurement throughput due to the need for high-homogeneity magnetic fields, necessitating sequential sample analysis, and expensive devices. Here, we propose a multichannel NMR device that addresses these limitations by leveraging the zero-to ultralow-field (ZULF) regime, where simultaneous detection of multiple samples is carried out via an array of compact optically...
Blake Andrews

Single-cell imaging of protein dynamics of paralogs reveals sources of gene retention

1 day 13 hours ago
Gene duplication is common across the Tree of Life and contributes to genomic robustness. In this study, we examined changes in the subcellular localization and abundance of proteins in response to the deletion of their paralogs originating from the whole-genome duplication event, which is a largely unexplored mechanism of functional divergence. We performed a systematic single-cell imaging analysis of protein dynamics and screened subcellular redistribution of proteins. We find that 20% of...
Rohan Dandage

Complete metagenome-assembled genome sequence of <em>Solidesulfovibrio</em> sp. DCME from a dichloromethane dechlorinating microbial community

1 day 13 hours ago
Here, we announce the closed genome of Solidesulfovibrio sp. DCME, assembled from metagenomic sequencing of an anaerobic dichloromethane mineralizing enrichment culture. The Solidesulfovibrio genus is known to cycle hydrogen, a key process for facilitating dichloromethane mineralization, which nominates this microbe as an important player in its microbial community.
Olivia Bulka

Gene expression landscape of the Brassica napus seed reveals subgenome bias in both space and time

3 days 13 hours ago
Brassica napus (canola; AnAnCnCn) contains both complete diploid genomes from its progenitors B. rapa (An) and B. oleracea (Cn). Despite growing knowledge of the gene expression landscape of the B. napus seed, little is known about subgenome bias underpinning the development of specific cells and tissues across the seed lifecycle. Here, we present a large-scale transcriptome atlas of the B. napus seed, including both the maternal seed coat and filial embryo and endosperm subregions. We report on...
Dylan J Ziegler

Multipotent lineage potential in B cell acute lymphoblastic leukemia is associated with distinct cellular origins and clinical features

4 days 13 hours ago
Developmental origins and their associations with lineage plasticity and treatment response in B-cell progenitor acute lymphoblastic leukemia (B-ALL) are mostly unexplored. Here, we integrated single-cell transcriptome sequencing (scRNA-seq) of 89 B-ALL samples with a single-cell atlas of normal human B cell development incorporating functional and molecular assays. We observed subtype- and sample-dependent correlation with normal developmental stage, with intra-subtype and intra-patient...
Ilaria Iacobucci

Targeting synthetic lethality between non-homologous end joining and radiation in very-high-risk medulloblastoma

6 days 13 hours ago
Specific and biologically informed treatments for medulloblastoma, especially for the highly lethal TP53-mutant SHH subgroup, remain elusive, where radiotherapy is the primary treatment modality. Leveraging genome-wide CRISPR-Cas9 dropout screening in combination with lethal doses of radiotherapy, we identify loss of p53 as the main driver of radiation resistance in SHH medulloblastoma. A negative-selection CRISPR-Cas9 screen across multiple models of Trp53-deficient SHH medulloblastoma reveals...
Alexandria DeCarlo

Fine-mapping genomic loci refines bipolar disorder risk genes

6 days 13 hours ago
Bipolar disorder is a heritable mental illness with complex etiology. While the largest published genome-wide association study identified 64 bipolar disorder risk loci, the causal SNPs and genes within these loci remain unknown. We applied a suite of statistical and functional fine-mapping methods to these loci and prioritized 17 likely causal SNPs for bipolar disorder. We mapped these SNPs to genes and investigated their likely functional consequences by integrating variant annotations, brain...
Maria Koromina

The practice of yoga and associations with embodiment, body image, internalized weight stigma, and disordered eating in a population-based sample of young adults

1 week ago
This study explores the prevalence of yoga practice and associations with embodiment, internalized weight stigma, body satisfaction, unhealthy weight control behaviors, and binge eating in a diverse population-based sample. Data were collected as part of the Eating and Activity over Time (EAT) study. Participants include 994 adults (mean age: 27.6; range: 24-35), with high percentages from marginalized ethnic/racial and low-income backgrounds. Cross-sectional and five-year longitudinal analyses...
Dianne Neumark-Sztainer

Risk factors for infection with SARS-CoV-2 in a cohort of Canadian healthcare workers: 2020-2023

1 week 1 day ago
Determining the factors that impact the risk for infection with SARS-CoV-2 is a priority as the virus continues to infect people worldwide. The objective was to determine the effectiveness of vaccines and other factors associated with infection among Canadian healthcare workers (HCWs) followed from 15 June 2020 to 1 December 2023. We also investigate the association between antibodies to SARS-CoV-2 and subsequent infections with SARS-CoV-2. Of the 2474 eligible participants, 2133 (86%) were...
Brenda L Coleman

Efficient slice anomaly detection network for 3D brain MRI Volume

1 week 4 days ago
Current anomaly detection methods excel with benchmark industrial data but struggle with natural images and medical data due to varying definitions of 'normal' and 'abnormal.' This makes accurate identification of deviations in these fields particularly challenging. Especially for 3D brain MRI data, all the state-of-the-art models are reconstruction-based with 3D convolutional neural networks which are memory-intensive, time-consuming and producing noisy outputs that require further...
Zeduo Zhang

CACHE Challenge #2: Targeting the RNA Site of the SARS-CoV-2 Helicase Nsp13

1 week 4 days ago
A critical assessment of computational hit-finding experiments (CACHE) challenge was conducted to predict ligands for the SARS-CoV-2 Nsp13 helicase RNA binding site, a highly conserved COVID-19 target. Twenty-three participating teams comprised of computational chemists and data scientists used protein structure and data from fragment-screening paired with advanced computational and machine learning methods to each predict up to 100 inhibitory ligands. Across all teams, 1957 compounds were...
Oleksandra Herasymenko

CompMap: an allele-specific expression read counter based on competitive mapping

1 week 5 days ago
Gene regulatory changes acting cis and trans to a gene can be inferred with allele-specific expression (ASE) transcriptomes from interspecies and inter-population hybrids and their parents. Problems of mapping bias and excessive information loss, however, can arise unintentionally from cumbersome analysis pipelines. We introduce CompMap, a self-contained method in Python that generates allele-specific expression counts from genotype-specific alignments. CompMap sorts and counts reads, not just...
Santiago Sanchez-Ramirez

Enhancing the applied force and range of axial optical tweezers

1 week 6 days ago
Axial optical tweezers provide a natural geometry for performing biomechanical assays, such as rupture force measurements of protein binding. Axial traps, however, are typically weaker than their lateral counterparts and require high laser power to maintain a well-calibrated, linear restoring force. Here, we show how to extend the spatial range over which well-calibrated forces can be applied by considering aberration effects and extend the range of applied forces by accounting for the nonlinear...
Zheng Zhang

Dissecting the Phenotypic Spectrum and Complexity of Movement Disorders in 22q11.2 Deletion Syndrome

1 week 6 days ago
CONCLUSIONS: We demonstrate that movement disorders in adults with 22q11.2DS exhibit greater clinical complexity than previously reported, which could reflect innate vulnerability and pathologic mechanisms beyond medication side effects. In those with a confirmed 22q11.2 microdeletion, periodic neurologic evaluations, supported by electrophysiologic investigations, enable accurate diagnosis and implementation of personalized management strategies.
Nikolai Gil D Reyes

Framework for Brain-Derived Dimensions of Psychopathology

1 week 6 days ago
CONCLUSION AND RELEVANCE: In this study, the identification of symptom groups of mental illness robustly defined by precisely characterized brain mechanisms enabled the characterization of dimensions of psychopathology based on quantifiable neurobiological measures.
Tristram A Lett

Line-scan imaging for real-time phenotypic screening of C. elegans

2 weeks ago
Phenotypic screens of living organisms often require real-time, high spatial resolution imaging. We report here on the design and application of a line-scan imaging platform that enables bright-field imaging of live nematodes using a 96-well plate configuration. The system optics are such that each individual well is projected across the full field of view of a 4 K line scanning camera. To minimize motional artifacts, the camera system is translated, while the sample is kept stationary. We...
Aaron Au
Checked
1 hour 11 minutes ago
Subscribe to Publications feed